AssayIndex
Chromatography

Chromophore

The part of a molecule responsible for UV absorbance. In peptides: the amide bond below 230 nm, and Trp/Tyr near 280 nm.

Related terms

Wavelength, detection
The UV wavelength at which absorbance is measured. 214 nm sees the amide backbone; 280 nm sees only aromatic residues. Purity figures at the two are not comparable.

Where this term appears

The glossary exists to make record pages readable without a chemistry degree. Every technical term on a report resolves here.