Terms beginning with T
10 of 238 glossary terms.
- Tailing factor (T)
- Peak shape measure at 5 % of peak height: (a + b)/2a. USP convention; related to but not identical with the asymmetry factor.
- Tangent skim
- Integrating a small peak on the trailing edge of a much larger one by drawing a tangential baseline. The convention above a height ratio of about 10:1.
- Trifluoroacetic acid (TFA)
- Mobile-phase additive at 0.1 %. Gives excellent peptide peak shape and suppresses electrospray ionisation. Also the most common peptide counter-ion.
- Time-of-flight (TOF)
- A mass analyser measuring the time for ions to traverse a flight tube. High resolving power; calibration is temperature-sensitive.
- Target-compound content
- Total peptide content multiplied by chromatographic purity. The fraction of the vial that is the named compound. The number that determines recovered mass.
- TFA adduct
- A trifluoroacetylated peptide, +95.982 Da, formed during cleavage or from prolonged exposure to trifluoroacetic acid.
- Truncation
- A peptide missing residues from one terminus. Distinguished from an internal deletion by the mass difference matching a contiguous run of residues.
- tBu residual
- Incompletely removed tert-butyl side-chain protection, +56.063 Da. Indicates a short or weak cleavage step.
- Threshold pyrogenic dose (K)
- 5.0 EU per kilogram per hour for parenteral routes, 0.2 EU/kg for intrathecal. Converting it to a per-milligram limit requires the dose.
- Tukey fence
- Q1 − 1.5 IQR and Q3 + 1.5 IQR. The convention for defining box-plot whiskers and outliers.
see also Asymmetry factor (As)
see also Drop-line integration
see also Counter-ion · Ion suppression
see also Resolving power
see also Peptide content · Recovered mass
see also Trifluoroacetic acid (TFA)
see also Deletion sequence
see also Fmoc
see also Bacterial endotoxin
see also Outlier