AssayIndex
Chromatography

Area percent

Purity expressed as the main peak's integrated area divided by the total integrated area of all peaks, times 100. A relative quantity. It is not a concentration and not a content.

Related terms

Assay
Quantification of an analyte against a material of known content. Distinct from area percent, which compares peaks within one chromatogram.
Peptide content
The fraction of total mass that is peptide, %w/w. Distinct from purity, which is a fraction of detected chromatographic area.
Relative response factor
The detector response of an impurity per unit mass, relative to the parent. Assumed to be 1.00 in almost all purity determinations, and usually is not.

Where this term appears

The glossary exists to make record pages readable without a chemistry degree. Every technical term on a report resolves here.