AssayIndex

AX-2026-042794

Thymulin · 5 mg declared · Nantong Guangyuan Chemical Co., Ltd. · lot GGPE-2510-C1982

PASSVERIFIED
Report ID
AX-2026-042794
Compound
Thymulin
Compound class
Tissue-repair and immunomodulatory peptides
Supplier
GGPeps — Nantong Guangyuan Chemical Co., Ltd.
Batch / lot
GGPE-2510-C1982
Declared label claim
5mg
Laboratory
VendorInvestigate
Assay date
05 Jan 2026
Submitted
10 Jan 2026
Quarter
2026-Q1
Submitter
j.lindholm
Sample provenance
Buyer-sourced
Section 1

Results

Measured against the index acceptance specification for the tissue-repair and immunomodulatory peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

ParameterMethodResultUnitIndex spec
PurityRP-HPLC, 32 min gradient, 214 nm98.45% area≥ 98.0PASS
Total impuritiesRP-HPLC, sum of reported peaks1.55% area≤ 2.0PASS
Largest single impurityRP-HPLC — deamidated (+1 Da)0.99% area≤ 1.00PASS
Identity — theoretical monoisotopic masscalculated from molecular formula873.3716Da—NOT TESTED
Identity — observed monoisotopic massLC-MS, high resolution (ESI-TOF/QTOF)873.3753Da—NOT TESTED
Mass accuracyobserved vs theoretical+4.2ppm± 15PASS
Peptide contentnot ordered on this submissionnot determined—required to check label claimNOT TESTED
Water contentKarl Fischer, coulometric, duplicate1.84%≤ 10.0PASS
Counter-ion (acetate)anion-exchange ion chromatography5.99%≤ 12.0PASS
Residual solvent + inorganic residueestimated — see /methods/residual-solvents-q3c/0.68%—NOT TESTED
Endotoxinkinetic chromogenic LAL0.50EU/mg≤ 5.0PASS
Sterilitynot ordered on this submissionnot determined—no growthNOT TESTED
Appearancevisual, diffuse light, black and white backgroundWhite cake, partial shrinkage from vial wall—white to off-white intact cakeFAIL
Reconstitution clarityreconstituted per submitter procedureVisible particulates, did not clear—clear, no visible particulatesFAIL
pH (reconstituted, 1 mg/mL)potentiometric, two-point calibration5.22—2.5 – 8.0PASS
Retention time (observed)RP-HPLC, 32 min gradient6.95min7.03 ± 0.25 (normalised)PASS
Peak asymmetry (10 % height)Ph. Eur. 2.2.461.22—0.80 – 1.50PASS
Plate count, main peakN = 5.54 (t/w½)²72,269—≥ 20,000PASS
Resolution to nearest impurityvs deamidated (+1 Da)1.81—no index criterionREPORTED
Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

0397179431191415885 Thymulin6.95 min · 98.45 % 6.76 same trace · ×50 vertical expansion, baseline-corrected · main peak off-scale reporting threshold 0.05 % area 05101520253035 Retention time (min) Response (mAU)
Figure 1. RP-HPLC trace, 32 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 72,269, and skew follows the reported asymmetry factor of 1.22. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.99 % impurity against a 98.45 % main peak is a line two pixels tall; the main peak runs off-scale there by design.
#PeakRTArea (µV·s)% areaΔ mass (Da)
1Thymulin — main peak6.954,784,97598.45—
2deamidated (+1 Da)6.7648,1170.99+0.984
3diketopiperazine (−18 Da)7.1811,1790.23-18.011
4des-Ser truncate (−87 Da)6.659,2350.19-87.032
5zinc-free apo-thymulin6.526,8040.14-63.929
Closure check — purity + Σ impurities 100.00CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 873.3716 Da, computed from C35H55N9O17. All ppm figures on this site are against monoisotopic values — see why that matters.

0255075100375475575675775875975 [M+1H]1+874.38[M+2H]2+437.69 m/z Relative abundance (%)
Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 873.3753 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.
ChargeTheoretical m/zObserved m/zΔ m/zRel. abundance (%)
2+437.6931437.69490.001855.5
1+874.3789874.38260.0037100.0
Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

FigureSupplier COAThis reportIndex note
Purity (% area)99.3698.45divergence +0.64 pp vs batch median
Total impurities (% area)0.641.55
Largest single impurity (% area)not stated0.99
Water (%)0.41.84
Counter-ion (%)not stated5.99
Peptide content (%w/w)not statednot determined
Endotoxin (EU/mg)not stated0.50
Method stated on certificateNO—no method section — the result cannot be reconciled against any other
Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance
Buyer-sourced
Evidentiary weight
Strongest available evidence in this index.
Chain of custody
Retail purchase → submitter (−20 °C, 2 d) → laboratory (courier, dry ice, 4 d) → assay.
Assay date
05 Jan 2026
Submitted to the index
10 Jan 2026
Laboratory
VendorInvestigate, Riga, Latvia
Method basis
32 min gradient · high-resolution MS
Verification status
VERIFIED
Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot GGPE-2510-C1982 n=4

ReportLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-042792GGPE-2510-C1982Janoshik2026-Q199.062.03104.2Supplier-supplied0PASS
AX-2026-042793GGPE-2510-C1982Janoshik2026-Q198.352.3191.4Supplier-supplied0PASS
AX-2026-042795GGPE-2510-C1982PeptideMeter2026-Q198.981.27—Supplier-supplied0PASS

Cross-sections

Other recent Thymulin records in the index.
ReportSupplierLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-041219WWBWWB-2602-S2129PeptideMeter2026-Q299.043.7989.7Supplier-supplied2MARGINAL
AX-2026-041216WWBWWB-2602-S2129Kestrel2026-Q299.464.0294.9Supplier-supplied1MARGINAL
AX-2026-041215WWBWWB-2602-S2129Nordanalyt2026-Q299.474.23—Supplier-supplied1MARGINAL
AX-2026-041218WWBWWB-2602-S2129Janoshik2026-Q299.634.40105.7Supplier-supplied1MARGINAL
AX-2026-041221WWBWWB-2602-S2129Nordanalyt2026-Q299.013.8692.6Supplier-supplied1MARGINAL
AX-2026-041223WWBWWB-2602-S2129Janoshik2026-Q299.173.78—Supplier-supplied1MARGINAL
AX-2026-041222WWBWWB-2602-S2129VendorInvestigate2026-Q298.763.36105.6Buyer-sourced1MARGINAL
AX-2026-041214WWBWWB-2602-S2129PeptideMeter2026-Q298.914.03—Supplier-supplied2MARGINAL
Other recent GGPeps records in the index.
ReportCompoundLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-042737SelankGGPE-2603-J9708Janoshik2026-Q398.409.1497.4Buyer-sourced0FAIL
AX-2026-042804SelankGGPE-2602-G2056Janoshik2026-Q398.507.97—Supplier-supplied0FAIL
AX-2026-042803SelankGGPE-2602-G2056Janoshik2026-Q398.488.01—Laboratory-retained1FAIL
AX-2026-042802SelankGGPE-2602-G2056Janoshik2026-Q398.747.0996.0Supplier-supplied0FAIL
AX-2026-042796SelankGGPE-2602-G2056Janoshik2026-Q398.267.5697.5Buyer-sourced0FAIL
AX-2026-042648Melanotan IIGGPE-2603-P8735Janoshik2026-Q399.106.97—Supplier-supplied4MARGINAL
AX-2026-042797SelankGGPE-2602-G2056Medutest2026-Q398.467.92—Buyer-sourced0FAIL
AX-2026-042669CJC-1295 (no DAC)GGPE-2606-E8495Medutest2026-Q398.614.0695.5Supplier-supplied0PASS