Retatrutide impurity profile
Every named species the index has reported alongside Retatrutide, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.59%
n=496
Median largest single
0.85%
n=496
Worst single observed
2.76%
n=496
Named species observed
8 / 8
n=8
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| [D-Aib2]-diastereomer | -0.22 | mass-identical | 92% | 0.21 | 2.26 |
| des-Aib20 analogue | -0.44 | -85.053 | 80% | 0.20 | 1.63 |
| C18 diacid regioisomer | +0.58 | mass-identical | 77% | 0.13 | 2.38 |
| truncated (5-39) | -1.92 | -428.201 | 73% | 0.14 | 2.76 |
| oxidised Trp (+16 Da) | -0.66 | +15.995 | 73% | 0.08 | 1.91 |
| aspartimide (−18 Da) | +0.71 | -18.011 | 62% | 0.10 | 1.65 |
| incomplete Fmoc removal (+222 Da) | +2.41 | +222.068 | 62% | 0.10 | 2.22 |
| retatrutide dimer | +4.12 | +4728.487 | 41% | 0.07 | 1.53 |
[D-Aib2]-diastereomer
- Retention offset from the main peak
- -0.22 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 456 of 496 records (92 %)
- Level when reported
- median 0.21 %, max 2.26 %
- Resolution requirement
- A gradient long enough to separate it. No mass spectrometer will.
des-Aib20 analogue
- Retention offset from the main peak
- -0.44 min on RG-2
- Mass difference
- -85.053 Da
- Reported in
- 399 of 496 records (80 %)
- Level when reported
- median 0.20 %, max 1.63 %
- Resolution requirement
- Baseline-resolved on the reference method.
C18 diacid regioisomer
- Retention offset from the main peak
- +0.58 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 383 of 496 records (77 %)
- Level when reported
- median 0.13 %, max 2.38 %
- Resolution requirement
- A gradient long enough to separate it. No mass spectrometer will.
truncated (5-39)
- Retention offset from the main peak
- -1.92 min on RG-2
- Mass difference
- -428.201 Da
- Reported in
- 361 of 496 records (73 %)
- Level when reported
- median 0.14 %, max 2.76 %
- Resolution requirement
- Baseline-resolved on the reference method.
oxidised Trp (+16 Da)
- Retention offset from the main peak
- -0.66 min on RG-2
- Mass difference
- +15.995 Da
- Reported in
- 361 of 496 records (73 %)
- Level when reported
- median 0.08 %, max 1.91 %
- Resolution requirement
- Baseline-resolved on the reference method.
aspartimide (−18 Da)
- Retention offset from the main peak
- +0.71 min on RG-2
- Mass difference
- -18.011 Da
- Reported in
- 308 of 496 records (62 %)
- Level when reported
- median 0.10 %, max 1.65 %
- Resolution requirement
- Baseline-resolved on the reference method.
incomplete Fmoc removal (+222 Da)
- Retention offset from the main peak
- +2.41 min on RG-2
- Mass difference
- +222.068 Da
- Reported in
- 306 of 496 records (62 %)
- Level when reported
- median 0.10 %, max 2.22 %
- Resolution requirement
- Baseline-resolved on the reference method.
retatrutide dimer
- Retention offset from the main peak
- +4.12 min on RG-2
- Mass difference
- +4728.487 Da
- Reported in
- 202 of 496 records (41 %)
- Level when reported
- median 0.07 %, max 1.53 %
- Resolution requirement
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- aspartimide formation and subsequent β-Asp isomerisation at Asp-Ser
- Trp oxidation under fluorescent light in clear glass
- aggregation above 2 mg/mL on freeze–thaw