AX-2025-019178
AOD-9604 · 10 mg declared · Tianjin Finder Chemical Co., Ltd. · lot TFC-2511-L5476
PASSPURITY ONLY
- Report ID
- AX-2025-019178
- Compound
- AOD-9604
- Compound class
- Metabolic and mitochondrial peptides
- Supplier
- TFC — Tianjin Finder Chemical Co., Ltd.
- Batch / lot
- TFC-2511-L5476
- Declared label claim
- 10mg
- Laboratory
- Medutest
- Assay date
- 02 Dec 2025
- Submitted
- 04 Dec 2025
- Quarter
- 2025-Q4
- Submitter
- d.wojcik
- Sample provenance
- Supplier-supplied
Section 1
Results
Measured against the index acceptance specification for the metabolic and mitochondrial peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.
| Parameter | Method | Result | Unit | Index spec | |
|---|---|---|---|---|---|
| Purity | RP-HPLC, 25 min gradient, 214 nm | 99.93 | % area | ≥ 98.0 | PASS |
| Total impurities | RP-HPLC, sum of reported peaks | 0.07 | % area | ≤ 2.0 | PASS |
| Largest single impurity | RP-HPLC — des-Tyr AOD (−163 Da) | 0.05 | % area | ≤ 1.00 | PASS |
| Identity | not ordered on this submission | not determined | — | required for VERIFIED status | NOT TESTED |
| Total peptide content | by difference from the gravimetric mass balance | 89.60 | %w/w | — | NOT TESTED |
| Target-compound content | total peptide content × purity | 89.54 | %w/w | — | NOT TESTED |
| Gross fill (as weighed) | five-place balance, under nitrogen | 11.76 | mg | 10 mg declared | NOT TESTED |
| Recovered target peptide | gross fill × target content | 10.530 | mg | 10 mg claim | NOT TESTED |
| Recovery against label claim | recovered ÷ claim | 105.3 | % | 90.0 – 110.0 | PASS |
| Water content | Karl Fischer, coulometric, duplicate | 3.02 | % | ≤ 9.0 | PASS |
| Counter-ion (acetate) | anion-exchange ion chromatography | 6.61 | % | ≤ 12.0 | PASS |
| Residual solvent + inorganic residue | estimated — see /methods/residual-solvents-q3c/ | 0.77 | % | — | NOT TESTED |
| Endotoxin | kinetic chromogenic LAL | 1.00 | EU/mg | ≤ 5.0 | PASS |
| Sterility | not ordered on this submission | not determined | — | no growth | NOT TESTED |
| Appearance | visual, diffuse light, black and white background | White cake, partially collapsed | — | white to off-white intact cake | FAIL |
| Reconstitution clarity | reconstituted per submitter procedure | Clear, colourless, no visible particulates | — | clear, no visible particulates | PASS |
| pH (reconstituted, 1 mg/mL) | potentiometric, two-point calibration | 5.07 | — | 2.5 – 8.0 | PASS |
| Retention time (observed) | RP-HPLC, 25 min gradient | 9.64 | min | 9.55 ± 0.30 (normalised) | PASS |
| Peak asymmetry (10 % height) | Ph. Eur. 2.2.46 | 1.39 | — | 0.80 – 1.50 | PASS |
| Plate count, main peak | N = 5.54 (t/w½)² | 55,622 | — | ≥ 20,000 | PASS |
| Resolution to nearest impurity | vs des-Tyr AOD (−163 Da) | 4.18 | — | no index criterion | REPORTED |
Section 2
Chromatogram and peak integration
The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.
| # | Peak | RT | Area (µV·s) | % area | Δ mass (Da) |
|---|---|---|---|---|---|
| 1 | AOD-9604 — main peak | 9.64 | 4,856,908 | 99.93 | — |
| 2 | des-Tyr AOD (−163 Da) | 8.94 | 2,430 | 0.05 | -163.063 |
| 3 | AOD dimer | 11.39 | 972 | 0.02 | +1813.852 |
| Closure check — purity + Σ impurities | 100.00 | CLOSES | |||
Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.
Section 4
Gravimetric mass balance
Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.
| Component | Determination | %w/w of cake |
|---|---|---|
| Water | Karl Fischer | 3.02 |
| Counter-ion (acetate) | ion chromatography | 6.61 |
| Residual solvent + inorganic residue | estimated | 0.77 |
| Total peptide content | by difference | 89.60 |
| Total | 100.00 CLOSES | |
Supplier certificate, as declared
The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.
| Figure | Supplier COA | This report | Index note |
|---|---|---|---|
| Purity (% area) | 99.90 | 99.93 | divergence +0.21 pp vs batch median |
| Total impurities (% area) | 0.10 | 0.07 | |
| Largest single impurity (% area) | not stated | 0.05 | |
| Water (%) | 3.1 | 3.02 | |
| Counter-ion (%) | not stated | 6.61 | |
| Peptide content (%w/w) | not stated | 89.60 | |
| Endotoxin (EU/mg) | 0.71 | 1.00 | |
| Method stated on certificate | YES | — | column, gradient and wavelength given |
Section 5
Provenance and caveats
Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.
- Sample provenance
- Supplier-supplied
- Evidentiary weight
- Weaker evidence. Read with the caveat below.
- Chain of custody
- Supplier despatch → laboratory direct (courier, cold pack, 7 d) → assay. Submitter did not handle the vial.
- Assay date
- 02 Dec 2025
- Submitted to the index
- 04 Dec 2025
- Laboratory
- Medutest, Ljubljana, Slovenia
- Method basis
- 25 min gradient · unit-resolution MS
- Verification status
- PURITY ONLY
Section 6
Annotations
No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.
Section 7
Related records
Same batch — lot TFC-2511-L5476 n=14
| Report | Lot | Lab | Quarter | Purity % | Water % | Recovery % | Provenance | Flags | Result |
|---|---|---|---|---|---|---|---|---|---|
| AX-2025-019173 | TFC-2511-L5476 | Janoshik | 2025-Q4 | 99.62 | 3.29 | 103.3 | Supplier-supplied | 1 | FAIL |
| AX-2025-019174 | TFC-2511-L5476 | Janoshik | 2025-Q4 | 99.93 | 3.59 | 99.7 | Supplier-supplied | 0 | PASS |
| AX-2025-019175 | TFC-2511-L5476 | Medutest | 2025-Q4 | 99.88 | 3.17 | — | Supplier-supplied | 0 | PASS |
| AX-2025-019176 | TFC-2511-L5476 | PeptideMeter | 2025-Q4 | 99.18 | 3.10 | — | Supplier-supplied | 0 | PASS |
| AX-2026-042001 | TFC-2511-L5476 | Janoshik | 2025-Q4 | 99.93 | 3.36 | 99.3 | Supplier-supplied | 0 | PASS |
| AX-2025-019177 | TFC-2511-L5476 | Janoshik | 2025-Q4 | 99.48 | 3.59 | 99.6 | Supplier-supplied | 0 | PASS |
| AX-2026-042002 | TFC-2511-L5476 | Kestrel | 2025-Q4 | 99.93 | 3.49 | 104.4 | Supplier-supplied | 0 | PASS |
| AX-2025-019179 | TFC-2511-L5476 | Nordanalyt | 2025-Q4 | 99.56 | 3.70 | 92.0 | Laboratory-retained | 0 | PASS |
| AX-2025-019180 | TFC-2511-L5476 | PeptideMeter | 2025-Q4 | 99.93 | 3.00 | — | Supplier-supplied | 0 | PASS |
| AX-2025-019181 | TFC-2511-L5476 | PeptideMeter | 2025-Q4 | 99.73 | 3.55 | 99.9 | Supplier-supplied | 0 | PASS |
| AX-2025-019182 | TFC-2511-L5476 | PeptideMeter | 2025-Q4 | 99.44 | 3.21 | 101.0 | Supplier-supplied | 0 | PASS |
| AX-2025-019183 | TFC-2511-L5476 | Kestrel | 2025-Q4 | 99.54 | 3.68 | — | Supplier-supplied | 0 | PASS |
| AX-2025-019184 | TFC-2511-L5476 | Janoshik | 2025-Q4 | 99.64 | 3.18 | 95.2 | Supplier-supplied | 0 | PASS |
Cross-sections
| Report | Supplier | Lot | Lab | Quarter | Purity % | Water % | Recovery % | Provenance | Flags | Result |
|---|---|---|---|---|---|---|---|---|---|---|
| AX-2026-042300 | HJ | HJ-2601-N2483 | Janoshik | 2026-Q2 | 98.77 | 5.32 | — | Buyer-sourced | 1 | MARGINAL |
| AX-2026-042297 | HJ | HJ-2601-N2483 | Medutest | 2026-Q2 | 99.00 | 4.72 | 99.5 | Supplier-supplied | 1 | MARGINAL |
| AX-2026-042299 | HJ | HJ-2601-N2483 | VendorInvestigate | 2026-Q2 | 98.14 | 5.21 | 103.7 | Buyer-sourced | 1 | MARGINAL |
| AX-2026-042296 | HJ | HJ-2601-N2483 | Janoshik | 2026-Q2 | 98.85 | 5.76 | 97.1 | Supplier-supplied | 1 | MARGINAL |
| AX-2026-042301 | HJ | HJ-2601-N2483 | VendorInvestigate | 2026-Q2 | 98.60 | 5.36 | 97.9 | Buyer-sourced | 1 | MARGINAL |
| AX-2026-042298 | HJ | HJ-2601-N2483 | Janoshik | 2026-Q2 | 99.02 | 5.14 | 96.5 | Supplier-supplied | 1 | MARGINAL |
| AX-2026-042295 | HJ | HJ-2601-N2483 | Medutest | 2026-Q2 | 98.88 | 5.80 | 93.8 | Supplier-supplied | 1 | MARGINAL |
| AX-2026-041474 | QYB | QYB-2508-E6017 | Janoshik | 2026-Q1 | 99.62 | 3.75 | — | Supplier-supplied | 0 | PASS |
| Report | Compound | Lot | Lab | Quarter | Purity % | Water % | Recovery % | Provenance | Flags | Result |
|---|---|---|---|---|---|---|---|---|---|---|
| AX-2026-041932 | Semaglutide | TFC-2604-X4301 | Janoshik | 2026-Q3 | 98.88 | 4.31 | — | Supplier-supplied | 0 | PASS |
| AX-2026-042067 | SS-31 (Elamipretide) | TFC-2604-Y7472 | Kestrel | 2026-Q3 | 98.66 | 4.44 | — | Supplier-supplied | 1 | MARGINAL |
| AX-2026-042057 | Sermorelin | TFC-2605-Y6440 | Janoshik | 2026-Q3 | 98.68 | 3.94 | 98.3 | Supplier-supplied | 0 | PASS |
| AX-2026-041936 | Semaglutide | TFC-2604-X4301 | PeptideMeter | 2026-Q3 | 98.62 | 4.30 | — | Buyer-sourced | 1 | MARGINAL |
| AX-2026-041973 | Humanin (HNG) | TFC-2605-U5564 | Janoshik | 2026-Q3 | 99.62 | 7.03 | — | Supplier-supplied | 2 | FAIL |
| AX-2026-041980 | Humanin (HNG) | TFC-2605-U5564 | Janoshik | 2026-Q3 | 99.90 | 7.01 | 88.6 | Supplier-supplied | 2 | MARGINAL |
| AX-2026-041961 | Tirzepatide | TFC-2605-G5134 | Medutest | 2026-Q3 | 98.25 | 7.11 | — | Supplier-supplied | 2 | MARGINAL |
| AX-2026-042068 | SS-31 (Elamipretide) | TFC-2604-Y7472 | Medutest | 2026-Q3 | 98.71 | 3.63 | 92.9 | Supplier-supplied | 2 | MARGINAL |