AssayIndex
Metabolic and mitochondrial peptides

MOTS-c

mitochondrial open reading frame of the 12S rRNA type-c · C101H152N28O22S2 · monoisotopic 2173.0918 Da. The index holds 112 assay reports on this compound across 21 batches from 12 suppliers.

112 reports21 batchesmedian 99.18 %index spec 98.0 %
Section 1

Analytical fingerprint

Hand-authored chemistry. These properties describe the substance and do not change when a record is added.

MOTS-c has two methionines and consequently a characteristic oxidation ladder: main peak, mono-sulfoxide at −0.38 min, bis-sulfoxide at −0.66 min. The ladder is diagnostic — if a report shows a single early impurity but no second rung, the sample was probably not oxidised and the peak is something else. The index's MOTS-c records show the highest sulfoxide levels of any short peptide it tracks, which is consistent with its being shipped without cold packs more often than not.

Molecular formula
C101H152N28O22S2
Monoisotopic mass
2173.0918Da
Average mass
2174.61Da
Residues
16
Nominal retention (RG-2, 30 min)
10.42min
Retention window
0.30min
Counter-ion as usually shipped
acetate
Typical label claims
5 mg · 10 mg
Analytical difficulty (index scale)
1.00 — straightforward
Purity is a defined quantity
YES
Section 2

Representative chromatogram

Drawn from this page's own medians. Not a scan, not an illustration, and not any single report.

025705141771110281 MOTS-c10.42 min · 99.18 % 9.76 same trace · ×200 vertical expansion, baseline-corrected · main peak off-scale reporting threshold 0.05 % area 05101520253035 Retention time (min) Response (mAU)
Figure 1. A representative MOTS-c trace on the index reference method RG-2, constructed from this page's own numbers: the main peak carries the index median purity of 99.18 % area at the nominal retention time of 10.42 min, and each impurity peak carries the median area the index has observed for that named species at its characteristic retention offset. It is not a scan of any single report — every individual record's own chromatogram is on its own page. The lower panel is the same trace at a stated vertical expansion, baseline-corrected, so the impurities are visible; the main peak runs off-scale there by design. Baseline noise is seeded from the compound slug.
Section 3

What the index has observed

Arithmetic over the 112 records the index holds. Aggregates on this page are computed over the records the index holds, which are not a random sample of the material on the market. Lots that get tested are lots someone chose to test. Read every figure here as a description of this dataset rather than as an estimate of a population.

Median purity 99.18% n=112
Interquartile range 1.26pp n=112
Minimum 96.96% n=112
Maximum 99.93% n=112
Pass rate vs spec 84.8% n=112
Median recovery vs claim 99.0% n=77
Median water 4.29% n=112
Median |mass error| 2.6ppm n=92
07142192.093.094.095.096.097.098.099.0100.092.00–92.25 %: n=092.25–92.50 %: n=092.50–92.75 %: n=092.75–93.00 %: n=093.00–93.25 %: n=093.25–93.50 %: n=093.50–93.75 %: n=093.75–94.00 %: n=094.00–94.25 %: n=094.25–94.50 %: n=094.50–94.75 %: n=094.75–95.00 %: n=095.00–95.25 %: n=095.25–95.50 %: n=095.50–95.75 %: n=095.75–96.00 %: n=096.00–96.25 %: n=096.25–96.50 %: n=096.50–96.75 %: n=096.75–97.00 %: n=197.00–97.25 %: n=197.25–97.50 %: n=297.50–97.75 %: n=397.75–98.00 %: n=1098.00–98.25 %: n=798.25–98.50 %: n=1198.50–98.75 %: n=1198.75–99.00 %: n=699.00–99.25 %: n=899.25–99.50 %: n=1799.50–99.75 %: n=1399.75–100.00 %: n=22median 99.18 Purity (% area) Reports
Figure 2. Purity distribution across all 112 MOTS-c records. Dashed line is this compound's median (99.18 %); the solid vertical is the index acceptance specification for the metabolic and mitochondrial peptides class (98.0 %). Domain is fixed at 92–100 % on every purity histogram on this site so that compounds can be compared by eye — a wide distribution here is a hard compound, a narrow one is an easy one, and neither is a statement about the suppliers.
Section 4

By supplier

Suppliers with fewer than two purity records on this compound are omitted from the figure rather than drawn as a line. The full table, including the thin cells, is on the by-supplier page.

96.797.397.998.499.099.6100.2SSASSA: Q1 97.84, median 98.33, Q3 99.16, n=27n=27GGPepsGGPeps: Q1 98.44, median 98.74, Q3 99.18, n=13n=13QSCQSC: Q1 99.65, median 99.93, Q3 99.93, n=13n=13BCHBCH: Q1 97.73, median 98.13, Q3 98.40, n=12n=12WWBWWB: Q1 99.33, median 99.64, Q3 99.84, n=12n=12TFCTFC: Q1 98.82, median 99.41, Q3 99.61, n=10n=10JEEPJEEP: Q1 99.34, median 99.67, Q3 99.76, n=8outlier 98.58n=8FGPFGP: Q1 99.65, median 99.73, Q3 99.89, n=8n=8WXTWXT: Q1 97.85, median 98.08, Q3 98.38, n=4n=4ERPERP: Q1 98.67, median 98.77, Q3 99.06, n=3n=3 Purity (% area)
Figure 3. MOTS-c purity by supplier, ordered by coverage. The vertical line is the index acceptance specification. Boxes that overlap are suppliers this dataset cannot separate, and most of them overlap.

Full per-supplier table with n= caveats →

Section 5

Characteristic impurities and degradants

The left three columns are authored chemistry — what this molecule characteristically carries and where it elutes. The right three are what the index has actually reported.

Named speciesΔRT (min, RG-2)Δ mass (Da)Seen inMedian % areaMax % area
des-Met1 (−131 Da)+0.22-131.04096%0.141.49
Met1 sulfoxide (+16 Da)-0.38+15.99594%0.191.86
bis-sulfoxide (+32 Da)-0.66+31.99090%0.241.83
deamidated (+1 Da)-0.30+0.98489%0.151.51

Degradation pathways

  • double methionine oxidation — MOTS-c carries two Met residues and both oxidise
  • N-terminal Met excision during expression-derived production

Full impurity profile, with what each named species implies about the synthesis →

Section 7

Quarterly coverage

QuarterReportsMedian purityRange
2024-Q10n<3—
2024-Q21n<3—
2024-Q31399.9399.42–99.93
2024-Q4899.7399.34–99.93
2025-Q11497.9297.34–99.11
2025-Q2598.1797.78–98.59
2025-Q33899.3898.41–99.93
2025-Q41398.7498.36–99.40
2026-Q1898.1297.59–98.81
2026-Q21298.1596.96–99.72
2026-Q30n<3—

Quarter-by-quarter detail →

Section 8

Most recent records

The 25 most recent MOTS-c records of 112.
ReportSupplierLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-041952TFCTFC-2605-R4770PeptideMeter2026-Q299.655.6095.5Supplier-supplied0PASS
AX-2026-043416WXTWXT-2511-P4848Janoshik2026-Q298.235.14106.7Supplier-supplied0PASS
AX-2026-043149SSASSA-2511-W7415Janoshik2026-Q298.005.42—Supplier-supplied0PASS
AX-2026-043498BCHBCH-2511-L5815Janoshik2026-Q296.963.7593.4Supplier-supplied0FAIL
AX-2026-043150SSASSA-2511-W7415Janoshik2026-Q298.335.76—Supplier-supplied0PASS
AX-2026-043152SSASSA-2511-W7415Janoshik2026-Q298.645.4888.9Supplier-supplied2MARGINAL
AX-2026-041953TFCTFC-2605-R4770Janoshik2026-Q299.725.2790.8Supplier-supplied2FAIL
AX-2026-043153SSASSA-2511-W7415Medutest2026-Q298.785.52—Supplier-supplied0PASS
AX-2026-043499BCHBCH-2511-L5815Medutest2026-Q297.043.58—Buyer-sourced0FAIL
AX-2026-043151SSASSA-2511-W7415Janoshik2026-Q297.586.06—Supplier-supplied0FAIL
AX-2026-043154SSASSA-2511-W7415Janoshik2026-Q297.766.0092.3Supplier-supplied0FAIL
AX-2026-043586QSTQST-2511-H7867PeptideMeter2026-Q298.065.1997.7Supplier-supplied0PASS
AX-2026-043431WXTWXT-2512-K4589Janoshik2026-Q198.812.1291.7Supplier-supplied1MARGINAL
AX-2026-043581BCHBCH-2601-S7929Janoshik2026-Q198.316.51—Supplier-supplied1MARGINAL
AX-2026-043583BCHBCH-2601-S7929PeptideMeter2026-Q197.846.60105.3Supplier-supplied0FAIL
AX-2026-043430WXTWXT-2512-K4589Janoshik2026-Q197.641.40101.6Supplier-supplied1FAIL
AX-2026-043582BCHBCH-2601-S7929PeptideMeter2026-Q197.596.19102.0Supplier-supplied0FAIL
AX-2026-043432WXTWXT-2512-K4589Janoshik2026-Q197.922.4790.0Supplier-supplied1FAIL
AX-2026-043584BCHBCH-2601-S7929Kestrel2026-Q198.506.4988.7Supplier-supplied2FAIL
AX-2026-043559BCHBCH-2601-U7218Janoshik2026-Q198.405.7693.3Supplier-supplied1MARGINAL
AX-2026-042805GGPepsGGPE-2508-J1903Janoshik2025-Q498.744.68100.0Supplier-supplied1MARGINAL
AX-2026-042806GGPepsGGPE-2508-J1903VendorInvestigate2025-Q498.713.7496.1Buyer-sourced1MARGINAL
AX-2025-020239GGPepsGGPE-2508-J1903Kestrel2025-Q499.375.08102.0Supplier-supplied0PASS
AX-2025-020234GGPepsGGPE-2508-J1903Janoshik2025-Q498.444.4193.2Supplier-supplied0PASS
AX-2025-020238GGPepsGGPE-2508-J1903Medutest2025-Q498.894.32—Supplier-supplied0PASS