AX-2026-043050 · Hexarelin · KP lot KP-2604-X5435

Section 1

Results

Measured against the index acceptance specification for the growth-hormone axis peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 25 min gradient, 214 nm	98.56	% area	≥ 99.0	FAIL
Total impurities	RP-HPLC, sum of reported peaks	1.44	% area	≤ 1.0	FAIL
Largest single impurity	RP-HPLC — C-terminal free acid (+1 Da)	0.57	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	886.4602	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, unit resolution (single quadrupole)	886.4554	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	-5.4	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	89.67	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	88.38	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	2.36	mg	2 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	2.086	mg	2 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	104.3	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	3.25	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	6.93	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.15	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	6.53	EU/mg	≤ 5.0	FAIL
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White cake, partially collapsed	—	white to off-white intact cake	FAIL
Reconstitution clarity	reconstituted per submitter procedure	Clear after 90 s gentle swirling, no particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.55	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 25 min gradient	7.62	min	7.55 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.12	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	62,316	—	≥ 20,000	PASS
Resolution to nearest impurity	vs C-terminal free acid (+1 Da)	1.07	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 25 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 62,316, and skew follows the reported asymmetry factor of 1.12. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.57 % impurity against a 98.56 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	Hexarelin — main peak	7.62	4,790,322	98.56	—
2	C-terminal free acid (+1 Da)	7.75	27,704	0.57	+0.984
3	des-methyl hexarelin (GHRP-6, −14 Da)	7.22	27,218	0.56	-14.016
4	des-Lys (−128 Da)	7.44	9,721	0.20	-128.095
5	oxidised Trp (+16 Da)	7.34	5,346	0.11	+15.995
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 886.4602 Da, computed from C47H58N12O6. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, unit-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 886.4554 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
2+	444.2374	444.2350	-0.0024	57.8
1+	887.4675	887.4627	-0.0048	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	3.25
Counter-ion (acetate)	ion chromatography	6.93
Residual solvent + inorganic residue	estimated	0.15
Total peptide content	by difference	89.67
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.0	98.56	divergence +0.45 pp vs batch median
Total impurities (% area)	1.00	1.44
Largest single impurity (% area)	0.50	0.57
Water (%)	1.2	3.25
Counter-ion (%)	4.0	6.93
Peptide content (%w/w)	94.2	89.67
Endotoxin (EU/mg)	not stated	6.53
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Supplier-supplied
Evidentiary weight: Weaker evidence. Read with the caveat below.
Chain of custody: Supplier despatch → laboratory direct (courier, cold pack, 3 d) → assay. Submitter did not handle the vial.
Assay date: 19 May 2026
Submitted to the index: 01 Jun 2026
Laboratory: Medutest, Ljubljana, Slovenia
Method basis: 25 min gradient · unit-resolution MS
Verification status: IDENTITY CONSISTENT

What this report does not establish

That the compound is not a near-isobaric alternative. Identity was confirmed at unit mass resolution, which cannot exclude species differing by 1 or 2 Da. The specific alternatives not excluded are named in the identity block above.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations 4

Signed technical comments from index contributors. Not a discussion forum — annotations question or contextualise the result, and the data team replies where a reply is warranted.

endotoxin_limit01 Jun 2026

Endotoxin burden at this level usually tracks water quality at the fill step rather than anything about the synthesis. A high-purity lot with high endotoxin is an entirely coherent result and this is one.

Contributor #P-118701 Jun 2026

Endotoxin undetectable means below the detection limit of on Medutest's system. That does not mean zero and may not reflect actual endotoxin. The cutoff is useful for screening; the absence of a number is not a guarantee.

oxytocin_pheur01 Jun 2026

6.53 EU/mg is elevated relative to the index median and the record shows no depyrogenation step. If this material will be reconstituted in aqueous buffer for any downstream use, that endotoxin load is a risk.

coa_template01 Jun 2026

Endotoxin assay but no bacterial viability or total count. The two are independent: dead endotoxin-bearing bacteria contribute to the burden, living ones that produce endotoxins do not.

Section 7

Related records

Same batch — lot KP-2604-X5435 n=10

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043043	KP-2604-X5435	VendorInvestigate	2026-Q2	98.54	3.33	107.2	Buyer-sourced	2	FAIL
AX-2026-043044	KP-2604-X5435	VendorInvestigate	2026-Q2	98.00	3.78	107.5	Buyer-sourced	2	FAIL
AX-2026-043045	KP-2604-X5435	Janoshik	2026-Q2	99.22	3.55	99.0	Supplier-supplied	2	MARGINAL
AX-2026-043046	KP-2604-X5435	Nordanalyt	2026-Q2	99.00	3.50	—	Supplier-supplied	2	MARGINAL
AX-2026-043047	KP-2604-X5435	Janoshik	2026-Q2	98.71	3.22	103.5	Supplier-supplied	2	FAIL
AX-2026-043048	KP-2604-X5435	VendorInvestigate	2026-Q2	98.28	3.15	94.2	Buyer-sourced	2	FAIL
AX-2026-043049	KP-2604-X5435	Nordanalyt	2026-Q2	98.46	3.64	103.0	Buyer-sourced	2	FAIL
AX-2026-043051	KP-2604-X5435	Janoshik	2026-Q2	99.10	3.16	104.0	Supplier-supplied	2	MARGINAL
AX-2026-043052	KP-2604-X5435	Janoshik	2026-Q2	97.98	2.79	—	Supplier-supplied	2	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent Hexarelin records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041892	JEEP	JEEP-2608-Y4367	Janoshik	2026-Q3	99.55	3.61	98.9	Supplier-supplied	1	MARGINAL
AX-2026-041893	JEEP	JEEP-2608-Y4367	PeptideMeter	2026-Q3	99.44	3.58	103.2	Supplier-supplied	0	PASS
AX-2026-043049	KP	KP-2604-X5435	Nordanalyt	2026-Q2	98.46	3.64	103.0	Buyer-sourced	2	FAIL
AX-2026-043279	CPC	CPC-2511-F1491	PeptideMeter	2026-Q2	98.87	5.35	—	Supplier-supplied	2	FAIL
AX-2026-042137	Homopeptide	HOMO-2602-R8195	Janoshik	2026-Q2	99.93	3.94	90.6	Supplier-supplied	1	MARGINAL
AX-2026-043048	KP	KP-2604-X5435	VendorInvestigate	2026-Q2	98.28	3.15	94.2	Buyer-sourced	2	FAIL
AX-2026-043274	CPC	CPC-2511-F1491	PeptideMeter	2026-Q2	98.89	5.10	—	Supplier-supplied	2	FAIL
AX-2026-043046	KP	KP-2604-X5435	Nordanalyt	2026-Q2	99.00	3.50	—	Supplier-supplied	2	MARGINAL

Other recent KP records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043029	Retatrutide	KP-2607-H5219	Medutest	2026-Q3	95.45	5.71	—	Supplier-supplied	2	FAIL
AX-2026-043030	Retatrutide	KP-2607-H5219	Kestrel	2026-Q3	95.97	6.95	97.1	Supplier-supplied	2	FAIL
AX-2026-043106	Tirzepatide	KP-2608-D5921	PeptideMeter	2026-Q3	98.90	5.45	105.4	Supplier-supplied	2	MARGINAL
AX-2026-043108	Tirzepatide	KP-2608-D5921	Janoshik	2026-Q3	99.70	5.45	—	Supplier-supplied	2	MARGINAL
AX-2026-043039	Retatrutide	KP-2607-H5219	Janoshik	2026-Q3	96.14	5.62	—	Supplier-supplied	2	FAIL
AX-2026-043100	Tirzepatide	KP-2608-D5921	VendorInvestigate	2026-Q3	98.27	5.31	94.4	Buyer-sourced	2	MARGINAL
AX-2026-043090	Ipamorelin	KP-2606-D5887	Janoshik	2026-Q3	98.18	3.07	101.2	Laboratory-retained	1	FAIL
AX-2026-043028	Retatrutide	KP-2607-H5219	Janoshik	2026-Q3	96.52	6.09	—	Supplier-supplied	2	MARGINAL