AX-2025-020772

N-Acetyl Semax Amide · 20 mg declared · Sangon Biotech (Shanghai) Co., Ltd. · lot SGN-2505-Y9398

PASSPURITY ONLY

Report ID: AX-2025-020772
Compound: N-Acetyl Semax Amide
Compound class: Neuroactive and cognitive peptides
Supplier: SGN — Sangon Biotech (Shanghai) Co., Ltd.
Batch / lot: SGN-2505-Y9398
Declared label claim: 20mg
Laboratory: PeptideMeter
Assay date: 06 Jul 2025
Submitted: 25 Jul 2025
Quarter: 2025-Q3
Submitter: s.trevino
Sample provenance: Buyer-sourced

Section 1

Results

Measured against the index acceptance specification for the neuroactive and cognitive peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 28 min gradient, 214 nm	99.26	% area	≥ 98.5	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.74	% area	≤ 1.5	PASS
Largest single impurity	RP-HPLC — semax (des-acetyl, des-amide, −41 Da)	0.34	% area	≤ 1.00	PASS
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Total peptide content	by difference from the gravimetric mass balance	87.52	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	86.87	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	23.29	mg	20 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	20.232	mg	20 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	101.2	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	6.77	%	≤ 12.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	4.88	%	≤ 10.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.83	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.49	EU/mg	≤ 5.0	PASS
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.75	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 28 min gradient	7.09	min	7.15 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	0.86	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	61,449	—	≥ 20,000	PASS
Resolution to nearest impurity	vs N-acetyl semax free acid (+1 Da)	1.75	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 28 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 61,449, and skew follows the reported asymmetry factor of 0.86. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.34 % impurity against a 99.26 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	N-Acetyl Semax Amide — main peak	7.09	4,824,344	99.26	—
2	semax (des-acetyl, des-amide, −41 Da)	6.57	16,525	0.34	-41.027
3	N-acetyl semax free acid (+1 Da)	7.30	10,207	0.21	+0.984
4	des-acetyl amide (−42 Da)	6.72	5,832	0.12	-42.011
5	Met sulfoxide (+16 Da)	6.77	3,402	0.07	+15.995
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	6.77
Counter-ion (acetate)	ion chromatography	4.88
Residual solvent + inorganic residue	estimated	0.83
Total peptide content	by difference	87.52
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.76	99.26	divergence +0.50 pp vs batch median
Total impurities (% area)	0.24	0.74
Largest single impurity (% area)	0.10	0.34
Water (%)	5.6	6.77
Counter-ion (%)	not stated	4.88
Peptide content (%w/w)	89.6	87.52
Endotoxin (EU/mg)	not stated	0.49
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Buyer-sourced
Evidentiary weight: Strongest available evidence in this index.
Chain of custody: Retail purchase → submitter (−20 °C, 20 d) → laboratory (courier, dry ice, 4 d) → assay.
Assay date: 06 Jul 2025
Submitted to the index: 25 Jul 2025
Laboratory: PeptideMeter, Tallinn, Estonia
Method basis: 28 min gradient · high-resolution MS
Verification status: PURITY ONLY

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot SGN-2505-Y9398 n=5

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Result
AX-2025-020773	SGN-2505-Y9398	VendorInvestigate	2025-Q3	98.81	7.00	—	Buyer-sourced	PASS
AX-2025-020774	SGN-2505-Y9398	Janoshik	2025-Q3	99.27	6.70	—	Supplier-supplied	PASS
AX-2025-020775	SGN-2505-Y9398	Medutest	2025-Q3	98.87	6.86	100.6	Buyer-sourced	PASS
AX-2025-020776	SGN-2505-Y9398	PeptideMeter	2025-Q3	99.47	6.72	—	Supplier-supplied	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent N-Acetyl Semax Amide records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043368	CPC	CPC-2602-D2941	Medutest	2026-Q3	98.79	6.05	102.1	Supplier-supplied	0	PASS
AX-2026-043574	BCH	BCH-2602-E8253	Janoshik	2026-Q3	97.75	8.85	97.9	Supplier-supplied	1	FAIL
AX-2026-043575	BCH	BCH-2602-E8253	Janoshik	2026-Q3	97.44	9.19	104.8	Supplier-supplied	1	FAIL
AX-2026-043367	CPC	CPC-2602-D2941	Nordanalyt	2026-Q3	99.39	6.16	92.4	Supplier-supplied	0	PASS
AX-2026-043573	BCH	BCH-2602-E8253	Janoshik	2026-Q3	97.47	8.59	—	Supplier-supplied	1	FAIL
AX-2026-043576	BCH	BCH-2602-E8253	Janoshik	2026-Q3	97.46	9.53	92.4	Laboratory-retained	1	FAIL
AX-2026-042746	GGPeps	GGPE-2512-A1206	PeptideMeter	2026-Q2	98.97	6.67	—	Supplier-supplied	2	MARGINAL
AX-2026-042234	Homopeptide	HOMO-2603-C1382	Janoshik	2026-Q2	98.99	8.00	99.6	Supplier-supplied	0	PASS

Other recent SGN records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043215	Afamelanotide	SGN-2602-Y9898	PeptideMeter	2026-Q3	98.92	3.40	—	Supplier-supplied	1	MARGINAL
AX-2026-043214	Afamelanotide	SGN-2602-Y9898	Janoshik	2026-Q3	99.19	3.41	106.9	Supplier-supplied	1	MARGINAL
AX-2026-043240	Melanotan II	SGN-2602-L1809	PeptideMeter	2026-Q2	98.65	2.87	—	Supplier-supplied	2	MARGINAL
AX-2026-043255	BPC-157	SGN-2512-H1950	Janoshik	2026-Q2	99.06	4.99	—	Supplier-supplied	2	MARGINAL
AX-2026-043242	Melanotan II	SGN-2602-L1809	VendorInvestigate	2026-Q2	98.14	2.18	107.2	Buyer-sourced	1	FAIL
AX-2026-043221	SLU-PP-332	SGN-2602-X9969	Kestrel	2026-Q2	98.70	5.87	—	Supplier-supplied	0	PASS
AX-2026-043245	Melanotan II	SGN-2602-L1809	Janoshik	2026-Q2	98.54	2.19	100.6	Supplier-supplied	2	MARGINAL
AX-2026-043244	Melanotan II	SGN-2602-L1809	Janoshik	2026-Q2	99.24	2.68	98.0	Supplier-supplied	1	MARGINAL