AX-2025-018896

MK-677 (Ibutamoren mesylate) · 1000 mg declared · Flourishglobal Peptide Biotech (Chengdu) Co., Ltd. · lot FGP-2502-F9841

FAILPURITY ONLY

Report ID: AX-2025-018896
Compound: MK-677 (Ibutamoren mesylate)
Compound class: Non-peptide adjuncts
Supplier: FGP — Flourishglobal Peptide Biotech (Chengdu) Co., Ltd.
Batch / lot: FGP-2502-F9841
Declared label claim: 1000mg
Laboratory: PeptideMeter
Assay date: 19 Apr 2025
Submitted: 03 May 2025
Quarter: 2025-Q2
Submitter: f.okwuosa
Sample provenance: Supplier-supplied

Section 1

Results

Measured against the index acceptance specification for the non-peptide adjuncts class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 28 min gradient, 214 nm	98.93	% area	≥ 98.5	PASS
Total impurities	RP-HPLC, sum of reported peaks	1.07	% area	≤ 1.5	PASS
Largest single impurity	RP-HPLC — free base (des-mesylate, −96 Da)	0.43	% area	≤ 1.00	PASS
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Total peptide content	by difference from the gravimetric mass balance	78.36	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	77.52	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	1296.17	mg	1000 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	1004.791	mg	1000 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	100.5	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	4.67	%	≤ 10.0	PASS
Counter-ion (mesylate)	anion-exchange ion chromatography	15.96	%	≤ 12.0	FAIL
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	1.01	%	—	NOT TESTED
Endotoxin	not ordered on this submission	not determined	—	≤ 5.0	NOT TESTED
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.97	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 28 min gradient	12.93	min	13.01 ± 0.30 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	0.86	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	82,712	—	≥ 20,000	PASS
Resolution to nearest impurity	vs free base (des-mesylate, −96 Da)	0.20	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 28 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 82,712, and skew follows the reported asymmetry factor of 0.86. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.43 % impurity against a 98.93 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	MK-677 (Ibutamoren mesylate) — main peak	12.93	4,808,305	98.93	—
2	free base (des-mesylate, −96 Da)	12.97	20,899	0.43	-96.011
3	sulfonamide hydrolysis product (+18 Da)	12.13	12,637	0.26	+18.011
4	residual DMF (process solvent)	5.28	11,179	0.23	-551.000
5	spiroindane regioisomer	13.34	4,860	0.10	0.000
6	O-desmethyl metabolite analogue (−14 Da)	12.44	2,430	0.05	-14.016
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	4.67
Counter-ion (mesylate)	ion chromatography	15.96
Residual solvent + inorganic residue	estimated	1.01
Total peptide content	by difference	78.36
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.90	98.93	divergence +0.42 pp vs batch median
Total impurities (% area)	not stated	1.07
Largest single impurity (% area)	not stated	0.43
Water (%)	2.1	4.67
Counter-ion (%)	not stated	15.96
Peptide content (%w/w)	93.1	78.36
Endotoxin (EU/mg)	not stated	not determined
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Supplier-supplied
Evidentiary weight: Weaker evidence. Read with the caveat below.
Chain of custody: Supplier despatch → laboratory direct (courier, cold pack, 2 d) → assay. Submitter did not handle the vial.
Assay date: 19 Apr 2025
Submitted to the index: 03 May 2025
Laboratory: PeptideMeter, Tallinn, Estonia
Method basis: 28 min gradient · high-resolution MS
Verification status: PURITY ONLY

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
That the material is free of bacterial endotoxin. Endotoxin was not measured. No chromatographic result bears on it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot FGP-2502-F9841 n=6

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2025-018893	FGP-2502-F9841	Janoshik	2025-Q2	99.47	3.71	91.1	Supplier-supplied	1	FAIL
AX-2025-018894	FGP-2502-F9841	Kestrel	2025-Q2	99.48	3.60	107.1	Supplier-supplied	1	FAIL
AX-2025-018895	FGP-2502-F9841	Medutest	2025-Q2	99.85	4.40	106.5	Supplier-supplied	1	FAIL
AX-2025-018897	FGP-2502-F9841	Nordanalyt	2025-Q2	99.74	4.08	—	Supplier-supplied	1	FAIL
AX-2025-018898	FGP-2502-F9841	Janoshik	2025-Q2	99.24	4.87	—	Supplier-supplied	1	FAIL

Batch rollup and reproducibility assessment →

Cross-sections

All MK-677 (Ibutamoren mesylate) records n=152
All FGP records n=296
All PeptideMeter records n=1107
All 2025-Q2 records n=556
MK-677 (Ibutamoren mesylate) by supplier
MK-677 (Ibutamoren mesylate) impurity profile

Other recent MK-677 (Ibutamoren mesylate) records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-043524	BCH	BCH-2511-G7276	Janoshik	2026-Q2	98.36	4.68	—	Supplier-supplied	1	FAIL
AX-2026-041764	FGP	FGP-2604-K1470	Janoshik	2026-Q2	99.93	5.34	—	Supplier-supplied	1	FAIL
AX-2026-043527	BCH	BCH-2511-G7276	Janoshik	2026-Q2	98.35	4.36	95.5	Supplier-supplied	1	FAIL
AX-2026-042409	SWB	SWB-2605-B4738	Medutest	2026-Q2	99.32	2.99	94.9	Laboratory-retained	1	FAIL
AX-2026-043526	BCH	BCH-2511-G7276	Medutest	2026-Q2	97.60	4.50	—	Buyer-sourced	1	FAIL
AX-2026-042410	SWB	SWB-2605-B4738	Janoshik	2026-Q2	99.61	2.81	—	Supplier-supplied	1	FAIL
AX-2026-041765	FGP	FGP-2604-K1470	Janoshik	2026-Q2	99.93	5.08	—	Supplier-supplied	0	FAIL
AX-2026-043525	BCH	BCH-2511-G7276	Medutest	2026-Q2	98.37	4.32	—	Buyer-sourced	1	FAIL

Other recent FGP records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041742	Tirzepatide	FGP-2603-L9714	Janoshik	2026-Q3	99.37	2.43	105.5	Supplier-supplied	1	MARGINAL
AX-2026-041767	Semaglutide	FGP-2602-M1508	PeptideMeter	2026-Q3	98.65	2.54	—	Supplier-supplied	2	FAIL
AX-2026-041741	Tirzepatide	FGP-2603-L9714	PeptideMeter	2026-Q3	98.62	2.57	—	Supplier-supplied	1	MARGINAL
AX-2026-041772	Semaglutide	FGP-2602-M1508	Janoshik	2026-Q3	99.79	3.03	107.5	Supplier-supplied	0	PASS
AX-2026-041770	Semaglutide	FGP-2602-M1508	VendorInvestigate	2026-Q3	99.33	1.48	90.3	Buyer-sourced	1	MARGINAL
AX-2026-041769	Semaglutide	FGP-2602-M1508	Janoshik	2026-Q3	99.13	2.55	92.9	Supplier-supplied	1	MARGINAL
AX-2026-041768	Semaglutide	FGP-2602-M1508	Janoshik	2026-Q3	98.85	2.20	90.3	Supplier-supplied	1	MARGINAL
AX-2026-041771	Semaglutide	FGP-2602-M1508	Janoshik	2026-Q3	99.41	2.31	—	Supplier-supplied	0	PASS