AX-2024-004874

NAD+ · 1000 mg declared · Shanghai Wibson Biotechnology Co., Ltd. · lot SWB-2405-L5945

MARGINALPARTIAL

Report ID: AX-2024-004874
Compound: NAD+
Compound class: Non-peptide adjuncts
Supplier: SWB — Shanghai Wibson Biotechnology Co., Ltd.
Batch / lot: SWB-2405-L5945
Declared label claim: 1000mg
Laboratory: Nordanalyt Laboratories
Assay date: 25 Jul 2024
Submitted: 31 Jul 2024
Quarter: 2024-Q3
Submitter: m.kimani
Sample provenance: Supplier-supplied

Section 1

Results

Measured against the index acceptance specification for the non-peptide adjuncts class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 35 min gradient, 214 nm	98.84	% area	≥ 97.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	1.16	% area	≤ 3.0	PASS
Largest single impurity	RP-HPLC — nicotinamide mononucleotide (NMN, −331 Da)	0.54	% area	≤ 1.00	PASS
Identity	not ordered on this submission	not determined	—	required for VERIFIED status	NOT TESTED
Total peptide content	by difference from the gravimetric mass balance	90.11	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	89.06	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	1009.24	mg	1000 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	898.829	mg	1000 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	89.9	%	90.0 – 110.0	FAIL
Water content	Karl Fischer, coulometric, duplicate	9.60	%	≤ 10.0	PASS
Counter-ion	no ionisable counter-ion expected	N/A	—	not applicable	NOT TESTED
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.29	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.47	EU/mg	≤ 5.0	PASS
Sterility	not ordered on this submission	not determined	—	no growth	NOT TESTED
Appearance	visual, diffuse light, black and white background	White powder, no defined cake (melt-back)	—	white to off-white intact cake	FAIL
Reconstitution clarity	reconstituted per submitter procedure	Clear, colourless, no visible particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	7.35	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 35 min gradient	4.79	min	4.80 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.08	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	44,070	—	≥ 20,000	PASS
Resolution to nearest impurity	vs α-NAD anomer	2.24	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 35 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 44,070, and skew follows the reported asymmetry factor of 1.08. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.54 % impurity against a 98.84 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	NAD+ — main peak	4.79	4,803,930	98.84	—
2	nicotinamide mononucleotide (NMN, −331 Da)	4.30	26,246	0.54	-331.058
3	ADP-ribose (−121 Da)	4.49	16,525	0.34	-121.040
4	NADH (reduced, +2 Da)	5.19	5,346	0.11	+2.016
5	nicotinamide (−541 Da)	4.00	4,374	0.09	-541.052
6	α-NAD anomer	5.00	3,888	0.08	0.000
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	9.60
Counter-ion	none expected	0.00
Residual solvent + inorganic residue	estimated	0.29
Total peptide content	by difference	90.11
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.31	98.84	divergence +0.34 pp vs batch median
Total impurities (% area)	0.69	1.16
Largest single impurity (% area)	not stated	0.54
Water (%)	7.8	9.60
Counter-ion (%)	not stated	N/A
Peptide content (%w/w)	not stated	90.11
Endotoxin (EU/mg)	0.34	0.47
Method stated on certificate	YES	—	column, gradient and wavelength given

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Supplier-supplied
Evidentiary weight: Weaker evidence. Read with the caveat below.
Chain of custody: Supplier despatch → laboratory direct (courier, cold pack, 6 d) → assay. Submitter did not handle the vial.
Assay date: 25 Jul 2024
Submitted to the index: 31 Jul 2024
Laboratory: Nordanalyt Laboratories, Uppsala, Sweden
Method basis: 35 min gradient · high-resolution MS
Verification status: PARTIAL

What this report does not establish

That the compound is the compound named. No identity confirmation was ordered on this submission. A purity figure describes chromatographic homogeneity of whatever eluted; it does not name it.
That the material is sterile. Sterility was not tested.
That any other vial in the same lot has the same composition. One vial was tested. Within-lot vial-to-vial variation in this index has a median of 0.28 pp and a 90th percentile of 0.74 pp.
That any other lot from the same supplier has the same composition. Batch-to-batch variation is larger than within-batch variation for every supplier here.
That the material is suitable for administration to a human or an animal. These are research compounds. No result on this site is a safety assessment and the index takes no position on use.
That the material was manufactured by the supplier that sold it. Contract synthesis is normal in this market and twenty-nine batches in the index fingerprint to a bulk from a different company than the one named on the label.

Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot SWB-2405-L5945 n=8

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2024-004873	SWB-2405-L5945	Medutest	2024-Q3	98.87	9.49	105.2	Supplier-supplied	1	FAIL
AX-2024-004875	SWB-2405-L5945	Janoshik	2024-Q3	99.19	9.10	—	Supplier-supplied	0	PASS
AX-2024-004876	SWB-2405-L5945	Janoshik	2024-Q3	99.18	9.29	—	Supplier-supplied	0	PASS
AX-2024-004877	SWB-2405-L5945	Nordanalyt	2024-Q3	98.52	9.87	100.2	Buyer-sourced	0	PASS
AX-2024-004878	SWB-2405-L5945	Janoshik	2024-Q3	99.07	9.30	100.0	Supplier-supplied	0	PASS
AX-2024-004879	SWB-2405-L5945	Medutest	2024-Q3	99.68	9.39	88.8	Supplier-supplied	2	FAIL
AX-2024-004880	SWB-2405-L5945	Janoshik	2024-Q3	98.65	9.07	91.4	Supplier-supplied	0	PASS

Batch rollup and reproducibility assessment →

Cross-sections

Other recent NAD+ records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-041208	WWB	WWB-2601-V1583	VendorInvestigate	2026-Q2	99.18	11.38	100.9	Buyer-sourced	0	FAIL
AX-2026-041209	WWB	WWB-2601-V1583	Nordanalyt	2026-Q2	99.82	11.64	—	Supplier-supplied	0	FAIL
AX-2026-041210	WWB	WWB-2601-V1583	Nordanalyt	2026-Q2	99.43	11.19	95.3	Supplier-supplied	0	FAIL
AX-2026-042457	SWB	SWB-2602-P5992	PeptideMeter	2026-Q2	99.06	10.72	96.8	Supplier-supplied	1	FAIL
AX-2026-041211	WWB	WWB-2601-V1583	Janoshik	2026-Q2	99.93	11.52	92.9	Supplier-supplied	0	FAIL
AX-2026-042456	SWB	SWB-2602-P5992	Medutest	2026-Q2	98.89	10.27	101.6	Supplier-supplied	2	FAIL
AX-2026-041213	WWB	WWB-2601-V1583	VendorInvestigate	2026-Q2	99.04	11.41	—	Buyer-sourced	0	FAIL
AX-2026-042454	SWB	SWB-2602-P5992	Janoshik	2026-Q2	98.64	11.21	—	Supplier-supplied	1	FAIL

Other recent SWB records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042461	Kisspeptin-10	SWB-2603-D6238	Janoshik	2026-Q3	98.35	4.65	96.5	Supplier-supplied	3	FAIL
AX-2026-042479	Melanotan II	SWB-2603-A5707	Janoshik	2026-Q3	99.16	3.67	94.5	Supplier-supplied	1	MARGINAL
AX-2026-042481	Melanotan II	SWB-2603-A5707	Medutest	2026-Q3	98.95	3.37	90.8	Supplier-supplied	1	MARGINAL
AX-2026-042460	Kisspeptin-10	SWB-2603-D6238	Kestrel	2026-Q3	99.55	4.21	90.8	Supplier-supplied	3	MARGINAL
AX-2026-042483	Melanotan II	SWB-2603-A5707	Janoshik	2026-Q3	99.38	3.73	107.2	Supplier-supplied	1	MARGINAL
AX-2026-042398	Semaglutide	SWB-2602-X5278	Nordanalyt	2026-Q3	99.08	4.48	—	Supplier-supplied	1	MARGINAL
AX-2026-042399	Semaglutide	SWB-2602-X5278	Janoshik	2026-Q3	98.56	3.82	—	Supplier-supplied	1	MARGINAL
AX-2026-042499	BPC-157	SWB-2607-Y5787	Kestrel	2026-Q3	99.93	4.25	94.4	Supplier-supplied	0	PASS