AX-2024-004744

GHRP-6 · 10 mg declared · Hunan Aslsen Technology Co., Ltd. · lot HOMO-2407-T9333

MARGINALVERIFIED

Report ID: AX-2024-004744
Compound: GHRP-6
Compound class: Growth-hormone axis peptides
Supplier: Homopeptide — Hunan Aslsen Technology Co., Ltd.
Batch / lot: HOMO-2407-T9333
Declared label claim: 10mg
Laboratory: Janoshik Analytical
Assay date: 04 Aug 2024
Submitted: 07 Aug 2024
Quarter: 2024-Q3
Submitter: h.adebayo
Sample provenance: Supplier-supplied

Section 1

Results

Measured against the index acceptance specification for the growth-hormone axis peptides class. A specification is a decision the index made; a result is a measurement. See where each threshold came from.

Parameter	Method	Result	Unit	Index spec
Purity	RP-HPLC, 30 min gradient, 214 nm	99.25	% area	≥ 99.0	PASS
Total impurities	RP-HPLC, sum of reported peaks	0.75	% area	≤ 1.0	PASS
Largest single impurity	RP-HPLC — oxidised Trp (+16 Da)	0.50	% area	≤ 1.00	PASS
Identity — theoretical monoisotopic mass	calculated from molecular formula	872.4446	Da	—	NOT TESTED
Identity — observed monoisotopic mass	LC-MS, high resolution (ESI-TOF/QTOF)	872.4469	Da	—	NOT TESTED
Mass accuracy	observed vs theoretical	+2.6	ppm	± 15	PASS
Total peptide content	by difference from the gravimetric mass balance	88.49	%w/w	—	NOT TESTED
Target-compound content	total peptide content × purity	87.83	%w/w	—	NOT TESTED
Gross fill (as weighed)	five-place balance, under nitrogen	11.22	mg	10 mg declared	NOT TESTED
Recovered target peptide	gross fill × target content	9.855	mg	10 mg claim	NOT TESTED
Recovery against label claim	recovered ÷ claim	98.6	%	90.0 – 110.0	PASS
Water content	Karl Fischer, coulometric, duplicate	6.97	%	≤ 9.0	PASS
Counter-ion (acetate)	anion-exchange ion chromatography	4.28	%	≤ 12.0	PASS
Residual solvent + inorganic residue	estimated — see /methods/residual-solvents-q3c/	0.26	%	—	NOT TESTED
Endotoxin	kinetic chromogenic LAL	0.17	EU/mg	≤ 5.0	PASS
Sterility	membrane filtration, USP <71>, 14 d	No growth at 14 d	—	no growth	PASS
Appearance	visual, diffuse light, black and white background	Off-white lyophilised cake, intact	—	white to off-white intact cake	PASS
Reconstitution clarity	reconstituted per submitter procedure	Clear after 90 s gentle swirling, no particulates	—	clear, no visible particulates	PASS
pH (reconstituted, 1 mg/mL)	potentiometric, two-point calibration	4.86	—	2.5 – 8.0	PASS
Retention time (observed)	RP-HPLC, 30 min gradient	8.39	min	8.44 ± 0.25 (normalised)	PASS
Peak asymmetry (10 % height)	Ph. Eur. 2.2.46	1.22	—	0.80 – 1.50	PASS
Plate count, main peak	N = 5.54 (t/w½)²	49,861	—	≥ 20,000	PASS
Resolution to nearest impurity	vs [D-Trp]-epimer	0.78	—	no index criterion	REPORTED

Section 2

Chromatogram and peak integration

The figure is drawn from the table. It is not an independent illustration — if a number in the table changed, the trace would change with it.

Figure 1. RP-HPLC trace, 30 min gradient, 214 nm. Drawn from the peak-integration table below: peak positions are the reported retention times, peak areas are the reported area percentages, peak widths follow the reported plate count of 49,861, and skew follows the reported asymmetry factor of 1.22. Baseline noise is seeded from the report ID and is illustrative. The lower panel is the same trace at a stated vertical expansion with the slow gradient drift subtracted, because at full scale a 0.50 % impurity against a 99.25 % main peak is a line two pixels tall; the main peak runs off-scale there by design.

#	Peak	RT	Area (µV·s)	% area	Δ mass (Da)
1	GHRP-6 — main peak	8.39	4,823,858	99.25	—
2	oxidised Trp (+16 Da)	8.05	24,302	0.50	+15.995
3	bis-oxidised Trp (+32 Da)	7.81	5,346	0.11	+31.990
4	[D-Trp]-epimer	8.27	3,402	0.07	0.000
5	des-Lys (−128 Da)	8.17	3,402	0.07	-128.095
Closure check — purity + Σ impurities				100.00	CLOSES

Reporting threshold 0.05 % area. Peaks below it are not reported and are therefore excluded from the total, which overstates purity by their sum — see peak integration.

Section 3

Mass spectrum and identity

Theoretical monoisotopic mass 872.4446 Da, computed from C46H56N12O6. All ppm figures on this site are against monoisotopic values — see why that matters.

Figure 2. ESI charge-state envelope, high-resolution instrument. Sticks are placed at m/z = (M + z × 1.007276)/z for the observed neutral mass of 872.4469 Da. Sodium (+21.982/z) and potassium (+37.956/z) adducts are shown where the parent charge state exceeds 25 % relative abundance; they are adducts of the same molecule and are not impurities.

Charge	Theoretical m/z	Observed m/z	Δ m/z	Rel. abundance (%)
2+	437.2296	437.2307	0.0011	57.5
1+	873.4519	873.4542	0.0023	100.0

Section 4

Gravimetric mass balance

Water, counter-ion, residual and total peptide content are exhaustive and mutually exclusive categories. They must account for 100 % of the cake mass. The index closes this to within 0.02 pp and the build asserts it.

Component	Determination	%w/w of cake
Water	Karl Fischer	6.97
Counter-ion (acetate)	ion chromatography	4.28
Residual solvent + inorganic residue	estimated	0.26
Total peptide content	by difference	88.49
Total		100.00 CLOSES

Supplier certificate, as declared

The supplier's own figures for this lot, beside the independent measurement. Divergence is assessed against the batch median, not against this single report — see rule AX-R05.

Figure	Supplier COA	This report	Index note
Purity (% area)	99.90	99.25	divergence +0.30 pp vs batch median
Total impurities (% area)	0.10	0.75
Largest single impurity (% area)	0.05	0.50
Water (%)	not stated	6.97
Counter-ion (%)	not stated	4.28
Peptide content (%w/w)	91.7	88.49
Endotoxin (EU/mg)	0.16	0.17
Method stated on certificate	NO	—	no method section — the result cannot be reconciled against any other

Section 5

Provenance and caveats

Where a sample came from changes the weight of the evidence. The index treats it as a first-class field.

Sample provenance: Supplier-supplied
Evidentiary weight: Weaker evidence. Read with the caveat below.
Chain of custody: Supplier despatch → laboratory direct (courier, cold pack, 5 d) → assay. Submitter did not handle the vial.
Assay date: 04 Aug 2024
Submitted to the index: 07 Aug 2024
Laboratory: Janoshik Analytical, Ostrava, Czechia
Method basis: 30 min gradient · high-resolution MS
Verification status: VERIFIED

Section 6

Annotations

No annotations on this record. Annotations are short signed technical comments from index contributors; see how to contribute.

Section 7

Related records

Same batch — lot HOMO-2407-T9333 n=3

Report	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2024-004743	HOMO-2407-T9333	Janoshik	2024-Q3	99.93	6.41	89.5	Supplier-supplied	1	MARGINAL
AX-2024-004745	HOMO-2407-T9333	Janoshik	2024-Q3	99.60	6.99	104.0	Supplier-supplied	1	MARGINAL

Batch rollup and reproducibility assessment →

Cross-sections

Other recent GHRP-6 records in the index.
Report	Supplier	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042290	HJ	HJ-2603-R2258	Janoshik	2026-Q3	99.71	3.05	—	Supplier-supplied	2	MARGINAL
AX-2026-042287	HJ	HJ-2603-R2258	VendorInvestigate	2026-Q3	98.79	2.43	90.9	Buyer-sourced	2	FAIL
AX-2026-042288	HJ	HJ-2603-R2258	Kestrel	2026-Q3	99.93	2.82	92.8	Supplier-supplied	2	MARGINAL
AX-2026-042289	HJ	HJ-2603-R2258	Janoshik	2026-Q3	99.53	2.67	104.7	Supplier-supplied	2	MARGINAL
AX-2026-042291	HJ	HJ-2603-R2258	Janoshik	2026-Q3	99.60	2.94	101.3	Supplier-supplied	2	MARGINAL
AX-2026-043560	BCH	BCH-2603-Y7018	Janoshik	2026-Q2	99.25	5.25	—	Supplier-supplied	1	MARGINAL
AX-2026-043564	BCH	BCH-2603-Y7018	Medutest	2026-Q2	98.44	4.83	—	Buyer-sourced	1	FAIL
AX-2026-043562	BCH	BCH-2603-Y7018	PeptideMeter	2026-Q2	98.57	4.52	97.9	Supplier-supplied	1	FAIL

Other recent Homopeptide records in the index.
Report	Compound	Lot	Lab	Quarter	Purity %	Water %	Recovery %	Provenance	Flags	Result
AX-2026-042181	Ipamorelin	HOMO-2608-A9045	Janoshik	2026-Q3	99.78	4.79	—	Supplier-supplied	1	MARGINAL
AX-2026-042172	Semaglutide	HOMO-2604-X8921	Janoshik	2026-Q3	99.64	5.16	91.9	Supplier-supplied	1	MARGINAL
AX-2026-042278	SLU-PP-332	HOMO-2602-Y2822	Kestrel	2026-Q3	99.71	4.42	—	Supplier-supplied	0	PASS
AX-2026-042180	Ipamorelin	HOMO-2608-A9045	Janoshik	2026-Q3	99.39	4.74	105.5	Supplier-supplied	1	MARGINAL
AX-2026-042184	Ipamorelin	HOMO-2608-A9045	Kestrel	2026-Q3	99.27	4.79	103.2	Supplier-supplied	1	MARGINAL
AX-2026-042274	SLU-PP-332	HOMO-2602-Y2822	VendorInvestigate	2026-Q3	98.71	4.72	—	Laboratory-retained	0	PASS
AX-2026-042183	Ipamorelin	HOMO-2608-A9045	VendorInvestigate	2026-Q3	99.38	4.97	—	Buyer-sourced	1	MARGINAL
AX-2026-042194	Retatrutide	HOMO-2605-V9833	PeptideMeter	2026-Q3	98.90	4.07	98.8	Supplier-supplied	3	MARGINAL