AssayIndex
Metabolic and mitochondrial peptides

Humanin (HNG)

HN · S14G-humanin · HNG · C119H182N30O32S · monoisotopic 2685.3153 Da. The index holds 42 assay reports on this compound across 8 batches from 7 suppliers.

42 reports8 batchesmedian 99.47 %index spec 97.5 %
Section 1

Analytical fingerprint

Hand-authored chemistry. These properties describe the substance and do not change when a record is added.

Two species are sold interchangeably as "humanin": the wild-type 24-mer and the S14G analogue HNG, which differ by 42 Da and 0.15 min. The index treats them as separate compounds and reclassifies on mass; roughly a fifth of submissions labelled HNG mass as wild type. Coverage is thin. Where a supplier's HNG lots repeatedly mass as wild type the supplier page notes it under documentation rather than under purity, because the material is not impure — it is mislabelled.

Molecular formula
C119H182N30O32S
Monoisotopic mass
2685.3153Da
Average mass
2687.00Da
Residues
24
Nominal retention (RG-2, 30 min)
12.14min
Retention window
0.30min
Counter-ion as usually shipped
acetate
Typical label claims
5 mg · 10 mg
Analytical difficulty (index scale)
1.05 — straightforward
Purity is a defined quantity
YES
Section 2

Representative chromatogram

Drawn from this page's own medians. Not a scan, not an illustration, and not any single report.

02213442566388850 Humanin (HNG)12.14 min · 99.47 % 10.79 same trace · ×200 vertical expansion, baseline-corrected · main peak off-scale reporting threshold 0.05 % area 05101520253035 Retention time (min) Response (mAU)
Figure 1. A representative Humanin (HNG) trace on the index reference method RG-2, constructed from this page's own numbers: the main peak carries the index median purity of 99.47 % area at the nominal retention time of 12.14 min, and each impurity peak carries the median area the index has observed for that named species at its characteristic retention offset. It is not a scan of any single report — every individual record's own chromatogram is on its own page. The lower panel is the same trace at a stated vertical expansion, baseline-corrected, so the impurities are visible; the main peak runs off-scale there by design. Baseline noise is seeded from the compound slug.
Section 3

What the index has observed

Arithmetic over the 42 records the index holds. Aggregates on this page are computed over the records the index holds, which are not a random sample of the material on the market. Lots that get tested are lots someone chose to test. Read every figure here as a description of this dataset rather than as an estimate of a population.

Median purity 99.47% n=42
Interquartile range 0.59pp n=42
Minimum 96.79% n=42
Maximum 99.93% n=42
Pass rate vs spec 97.6% n=42
Median recovery vs claim 97.6% n=26
Median water 5.07% n=42
Median |mass error| 2.8ppm n=33
036992.093.094.095.096.097.098.099.0100.092.00–92.25 %: n=092.25–92.50 %: n=092.50–92.75 %: n=092.75–93.00 %: n=093.00–93.25 %: n=093.25–93.50 %: n=093.50–93.75 %: n=093.75–94.00 %: n=094.00–94.25 %: n=094.25–94.50 %: n=094.50–94.75 %: n=094.75–95.00 %: n=095.00–95.25 %: n=095.25–95.50 %: n=095.50–95.75 %: n=095.75–96.00 %: n=096.00–96.25 %: n=096.25–96.50 %: n=096.50–96.75 %: n=096.75–97.00 %: n=197.00–97.25 %: n=097.25–97.50 %: n=097.50–97.75 %: n=097.75–98.00 %: n=098.00–98.25 %: n=098.25–98.50 %: n=398.50–98.75 %: n=098.75–99.00 %: n=599.00–99.25 %: n=699.25–99.50 %: n=999.50–99.75 %: n=999.75–100.00 %: n=9median 99.47 Purity (% area) Reports
Figure 2. Purity distribution across all 42 Humanin (HNG) records. Dashed line is this compound's median (99.47 %); the solid vertical is the index acceptance specification for the metabolic and mitochondrial peptides class (97.5 %). Domain is fixed at 92–100 % on every purity histogram on this site so that compounds can be compared by eye — a wide distribution here is a hard compound, a narrow one is an easy one, and neither is a statement about the suppliers.
Section 4

By supplier

Suppliers with fewer than two purity records on this compound are omitted from the figure rather than drawn as a line. The full table, including the thin cells, is on the by-supplier page.

98.298.598.899.299.599.8100.1TFCTFC: Q1 99.41, median 99.68, Q3 99.91, n=12outlier 98.40outlier 98.46n=12HomopeptideHomopeptide: Q1 99.51, median 99.71, Q3 99.84, n=11n=11GGPepsGGPeps: Q1 99.14, median 99.22, Q3 99.44, n=7outlier 99.93n=7ERPERP: Q1 98.78, median 98.88, Q3 99.25, n=6n=6QYBQYB: Q1 99.31, median 99.47, Q3 99.56, n=3n=3SSASSA: Q1 98.95, median 99.07, Q3 99.19, n=2n=2 Purity (% area)
Figure 3. Humanin (HNG) purity by supplier, ordered by coverage. The vertical line is the index acceptance specification. Boxes that overlap are suppliers this dataset cannot separate, and most of them overlap.

Full per-supplier table with n= caveats →

Section 5

Characteristic impurities and degradants

The left three columns are authored chemistry — what this molecule characteristically carries and where it elutes. The right three are what the index has actually reported.

Named speciesΔRT (min, RG-2)Δ mass (Da)Seen inMedian % areaMax % area
wild-type humanin (Ser14, −42 Da)-0.15-42.011100%0.100.95
deamidated (+1 Da)-0.28+0.98488%0.121.17
truncated (1-20)-1.35-472.24588%0.110.89
Met sulfoxide (+16 Da)-0.42+15.99586%0.071.59

Degradation pathways

  • Met oxidation
  • aggregation above 1 mg/mL in neutral buffer

Full impurity profile, with what each named species implies about the synthesis →

Section 7

Quarterly coverage

QuarterReportsMedian purityRange
2024-Q10n<3—
2024-Q2899.1996.79–99.93
2024-Q30n<3—
2024-Q40n<3—
2025-Q11199.7199.21–99.93
2025-Q20n<3—
2025-Q32n<398.84–99.30
2025-Q40n<3—
2026-Q1999.1498.37–99.64
2026-Q20n<3—
2026-Q31299.6898.40–99.93

Quarter-by-quarter detail →

Section 8

Most recent records

The 25 most recent Humanin (HNG) records of 42.
ReportSupplierLotLabQuarterPurity %Water %Recovery %ProvenanceFlagsResult
AX-2026-041973TFCTFC-2605-U5564Janoshik2026-Q399.627.03—Supplier-supplied2FAIL
AX-2026-041980TFCTFC-2605-U5564Janoshik2026-Q399.907.0188.6Supplier-supplied2MARGINAL
AX-2026-041979TFCTFC-2605-U5564Nordanalyt2026-Q399.736.64—Supplier-supplied1MARGINAL
AX-2026-041981TFCTFC-2605-U5564Medutest2026-Q399.876.84—Supplier-supplied1MARGINAL
AX-2026-041975TFCTFC-2605-U5564Nordanalyt2026-Q399.936.8691.5Supplier-supplied1MARGINAL
AX-2026-041977TFCTFC-2605-U5564Medutest2026-Q399.196.76103.8Supplier-supplied1MARGINAL
AX-2026-041972TFCTFC-2605-U5564Janoshik2026-Q399.486.1192.0Supplier-supplied1MARGINAL
AX-2026-041974TFCTFC-2605-U5564Janoshik2026-Q399.936.3196.6Supplier-supplied1MARGINAL
AX-2026-041978TFCTFC-2605-U5564Janoshik2026-Q399.496.1796.5Supplier-supplied1MARGINAL
AX-2026-041955TFCTFC-2608-S5204Janoshik2026-Q398.461.92—Supplier-supplied1MARGINAL
AX-2026-041954TFCTFC-2608-S5204Janoshik2026-Q398.401.7392.0Supplier-supplied1MARGINAL
AX-2026-041976TFCTFC-2605-U5564Medutest2026-Q399.936.69—Supplier-supplied1MARGINAL
AX-2026-042506ERPERP-2601-P6603Janoshik2026-Q198.373.83—Supplier-supplied1MARGINAL
AX-2026-042505ERPERP-2601-P6603Janoshik2026-Q199.353.74101.7Supplier-supplied1MARGINAL
AX-2026-042507ERPERP-2601-P6603Janoshik2026-Q198.793.07107.3Supplier-supplied1MARGINAL
AX-2026-041471QYBQYB-2512-A5720Nordanalyt2026-Q199.474.0697.9Supplier-supplied1MARGINAL
AX-2026-041469QYBQYB-2512-A5720Medutest2026-Q199.644.7097.2Supplier-supplied2FAIL
AX-2026-042503ERPERP-2601-P6603Medutest2026-Q198.773.55—Supplier-supplied1MARGINAL
AX-2026-041470QYBQYB-2512-A5720Kestrel2026-Q199.145.0493.4Supplier-supplied2FAIL
AX-2026-042504ERPERP-2601-P6603Janoshik2026-Q199.454.32100.7Supplier-supplied1MARGINAL
AX-2026-042502ERPERP-2601-P6603Medutest2026-Q198.974.1991.5Supplier-supplied1MARGINAL
AX-2025-020555SSASSA-2508-P6816PeptideMeter2025-Q398.845.10107.5Supplier-supplied1MARGINAL
AX-2025-020556SSASSA-2508-P6816Janoshik2025-Q399.304.89101.3Supplier-supplied1MARGINAL
AX-2025-019510HomopeptideHOMO-2408-A1230Janoshik2025-Q199.575.05105.7Supplier-supplied1MARGINAL
AX-2025-019509HomopeptideHOMO-2408-A1230PeptideMeter2025-Q199.935.15—Supplier-supplied1MARGINAL