SS-31 (Elamipretide) impurity profile
Every named species the index has reported alongside SS-31 (Elamipretide), how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.58%
n=97
Median largest single
0.33%
n=97
Worst single observed
1.89%
n=97
Named species observed
4 / 4
n=4
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| C-terminal free acid (+1 Da) | +0.20 | +0.984 | 95% | 0.08 | 1.89 |
| des-Dmt (−193 Da) | -0.72 | -193.110 | 88% | 0.11 | 1.88 |
| [D-Arg1]-epimer | -0.18 | mass-identical | 79% | 0.13 | 1.34 |
| acetyl adduct (+42 Da) | +0.54 | +42.011 | 78% | 0.24 | 1.31 |
C-terminal free acid (+1 Da)
- Retention offset from the main peak
- +0.20 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 92 of 97 records (95 %)
- Level when reported
- median 0.08 %, max 1.89 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
des-Dmt (−193 Da)
- Retention offset from the main peak
- -0.72 min on RG-2
- Mass difference
- -193.110 Da
- Reported in
- 85 of 97 records (88 %)
- Level when reported
- median 0.11 %, max 1.88 %
- Resolution requirement
- Baseline-resolved on the reference method.
[D-Arg1]-epimer
- Retention offset from the main peak
- -0.18 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 77 of 97 records (79 %)
- Level when reported
- median 0.13 %, max 1.34 %
- Resolution requirement
- A gradient long enough to separate it. No mass spectrometer will.
acetyl adduct (+42 Da)
- Retention offset from the main peak
- +0.54 min on RG-2
- Mass difference
- +42.011 Da
- Reported in
- 76 of 97 records (78 %)
- Level when reported
- median 0.24 %, max 1.31 %
- Resolution requirement
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- C-terminal amide hydrolysis
- epimerisation at Arg1 under prolonged basic coupling