AssayIndex
Cross-section

Glucagon by laboratory

The same compound measured by different laboratories on different gradients. Where the medians differ, the question is whether the material differed or the method did — and for Glucagon, with a class minimum gradient of 25 min, the method is a live explanation.

10 recordsmedian 98.78 %spec 98.0 %
LaboratoryReportsGradientMS resolutionMedian purity %Published bias (pp)Bias-adjustedMethod suitability
Janoshik4 n=430minHigh98.27+0.0098.27ADEQUATE
PeptideMeter2 n=228minHigh98.91, 99.21-0.05—ADEQUATE
VendorInvestigate2 n=232minHigh98.15, 97.91-0.11—ADEQUATE
Nordanalyt2 n=235minHigh99.85, 99.32+0.02—ADEQUATE
Medutest025minUnit—+0.08—ADEQUATE
Kestrel015minUnit—+0.24—BELOW CLASS MINIMUM
97.698.098.498.899.299.6100.0JanoshikJanoshik: Q1 97.87, median 98.27, Q3 98.77, n=4n=4PeptideMeterPeptideMeter: Q1 98.98, median 99.06, Q3 99.13, n=2n=2VendorInvestigateVendorInvestigate: Q1 97.97, median 98.03, Q3 98.09, n=2n=2NordanalytNordanalyt: Q1 99.45, median 99.58, Q3 99.72, n=2n=2 Purity (% area)
Figure. Glucagon purity by contributing laboratory. Any offset between rows has two possible sources — a real difference in the material each laboratory happened to receive, and the laboratory's own bias — and this figure cannot separate them. The paired analysis that can is on inter-lab agreement.