Oxytocin impurity profile
Every named species the index has reported alongside Oxytocin, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.43%
n=14
Median largest single
0.17%
n=14
Worst single observed
0.83%
n=14
Named species observed
5 / 5
n=5
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| open-chain (reduced disulfide, +2 Da) | -0.52 | +2.016 | 86% | 0.08 | 0.83 |
| des-Gly9 (−57 Da) | -0.30 | -57.021 | 79% | 0.07 | 0.17 |
| deamidated Gln4 (+1 Da) | -0.22 | +0.984 | 64% | 0.14 | 0.30 |
| oxytocin dimer (intermolecular disulfide) | +2.18 | +1004.421 | 57% | 0.06 | 0.14 |
| Tyr sulfoxide artefact (+16 Da) | -0.28 | +15.995 | 50% | 0.05 | 0.57 |
open-chain (reduced disulfide, +2 Da)
- Retention offset from the main peak
- -0.52 min on RG-2
- Mass difference
- +2.016 Da
- Reported in
- 12 of 14 records (86 %)
- Level when reported
- median 0.08 %, max 0.83 %
- Resolution requirement
- Baseline-resolved on the reference method.
des-Gly9 (−57 Da)
- Retention offset from the main peak
- -0.30 min on RG-2
- Mass difference
- -57.021 Da
- Reported in
- 11 of 14 records (79 %)
- Level when reported
- median 0.07 %, max 0.17 %
- Resolution requirement
- Baseline-resolved on the reference method.
deamidated Gln4 (+1 Da)
- Retention offset from the main peak
- -0.22 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 9 of 14 records (64 %)
- Level when reported
- median 0.14 %, max 0.30 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
oxytocin dimer (intermolecular disulfide)
- Retention offset from the main peak
- +2.18 min on RG-2
- Mass difference
- +1004.421 Da
- Reported in
- 8 of 14 records (57 %)
- Level when reported
- median 0.06 %, max 0.14 %
- Resolution requirement
- Baseline-resolved on the reference method.
Tyr sulfoxide artefact (+16 Da)
- Retention offset from the main peak
- -0.28 min on RG-2
- Mass difference
- +15.995 Da
- Reported in
- 7 of 14 records (50 %)
- Level when reported
- median 0.05 %, max 0.57 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- disulfide reduction then dimerisation
- Gln4 deamidation — Ph. Eur. limits this specifically