AssayIndex
Cross-section

Melanotan II impurity profile

Every named species the index has reported alongside Melanotan II, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.

186 recordsmedian 99.24 %spec 98.5 %
Median total impurities 0.76% n=186
Median largest single 0.36% n=186
Worst single observed 2.11% n=186
Named species observed 5 / 5 n=5
Named speciesΔRT (min)Δ mass (Da)Seen inMedian % areaMax % area
[L-Phe7]-diastereomer-0.16mass-identical92%0.080.98
linear (open-lactam) precursor (+18 Da)-0.74+18.01191%0.211.19
oxidised Trp (+16 Da)-0.36+15.99587%0.092.11
des-acetyl MT-II (−42 Da)-0.48-42.01185%0.091.14
MT-II dimer (head-to-tail)+2.35+1023.54071%0.060.82

[L-Phe7]-diastereomer

Retention offset from the main peak
-0.16 min on RG-2
Mass difference
mass-identical — chromatography only
Reported in
171 of 186 records (92 %)
Level when reported
median 0.08 %, max 0.98 %
Resolution requirement
A gradient long enough to separate it. No mass spectrometer will.

linear (open-lactam) precursor (+18 Da)

Retention offset from the main peak
-0.74 min on RG-2
Mass difference
+18.011 Da
Reported in
169 of 186 records (91 %)
Level when reported
median 0.21 %, max 1.19 %
Resolution requirement
Baseline-resolved on the reference method.

oxidised Trp (+16 Da)

Retention offset from the main peak
-0.36 min on RG-2
Mass difference
+15.995 Da
Reported in
162 of 186 records (87 %)
Level when reported
median 0.09 %, max 2.11 %
Resolution requirement
Baseline-resolved on the reference method.

des-acetyl MT-II (−42 Da)

Retention offset from the main peak
-0.48 min on RG-2
Mass difference
-42.011 Da
Reported in
159 of 186 records (85 %)
Level when reported
median 0.09 %, max 1.14 %
Resolution requirement
Baseline-resolved on the reference method.

MT-II dimer (head-to-tail)

Retention offset from the main peak
+2.35 min on RG-2
Mass difference
+1023.540 Da
Reported in
132 of 186 records (71 %)
Level when reported
median 0.06 %, max 0.82 %
Resolution requirement
Baseline-resolved on the reference method.

Degradation pathways

Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.

  • Trp oxidation
  • lactam hydrolysis reopening the ring