CJC-1295 (no DAC) impurity profile
Every named species the index has reported alongside CJC-1295 (no DAC), how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
1.39%
n=137
Median largest single
0.69%
n=137
Worst single observed
2.00%
n=137
Named species observed
5 / 5
n=5
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| des-Tyr1 (−163 Da) | -0.54 | -163.063 | 98% | 0.32 | 1.77 |
| native GRF (1-29) (−59 Da) | -0.34 | -58.995 | 94% | 0.21 | 2.00 |
| deamidated (+1 Da) | -0.24 | +0.984 | 93% | 0.17 | 1.33 |
| truncated (1-24) | -1.16 | -570.291 | 92% | 0.11 | 1.28 |
| acetyl adduct (+42 Da) | +0.44 | +42.011 | 88% | 0.08 | 1.13 |
des-Tyr1 (−163 Da)
- Retention offset from the main peak
- -0.54 min on RG-2
- Mass difference
- -163.063 Da
- Reported in
- 134 of 137 records (98 %)
- Level when reported
- median 0.32 %, max 1.77 %
- Resolution requirement
- Baseline-resolved on the reference method.
native GRF (1-29) (−59 Da)
- Retention offset from the main peak
- -0.34 min on RG-2
- Mass difference
- -58.995 Da
- Reported in
- 129 of 137 records (94 %)
- Level when reported
- median 0.21 %, max 2.00 %
- Resolution requirement
- Baseline-resolved on the reference method.
deamidated (+1 Da)
- Retention offset from the main peak
- -0.24 min on RG-2
- Mass difference
- +0.984 Da
- Reported in
- 127 of 137 records (93 %)
- Level when reported
- median 0.17 %, max 1.33 %
- Resolution requirement
- Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.
truncated (1-24)
- Retention offset from the main peak
- -1.16 min on RG-2
- Mass difference
- -570.291 Da
- Reported in
- 126 of 137 records (92 %)
- Level when reported
- median 0.11 %, max 1.28 %
- Resolution requirement
- Baseline-resolved on the reference method.
acetyl adduct (+42 Da)
- Retention offset from the main peak
- +0.44 min on RG-2
- Mass difference
- +42.011 Da
- Reported in
- 121 of 137 records (88 %)
- Level when reported
- median 0.08 %, max 1.13 %
- Resolution requirement
- Baseline-resolved on the reference method.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- Asn8 deamidation — the reason the D-Ala/Gln/Ala/Leu substitutions exist
- Asp-Gly aspartimide during synthesis