AssayIndex
Cross-section

CJC-1295 with DAC impurity profile

Every named species the index has reported alongside CJC-1295 with DAC, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.

136 recordsmedian 98.79 %spec 97.5 %
Median total impurities 1.21% n=136
Median largest single 0.59% n=136
Worst single observed 2.10% n=136
Named species observed 4 / 4 n=4
Named speciesΔRT (min)Δ mass (Da)Seen inMedian % areaMax % area
hydrolysed maleimide (+18 Da)-0.62+18.01199%0.352.10
maleimide dimer+2.42+3644.97596%0.151.71
mod-GRF (1-29) (−279 Da)-1.76-279.23594%0.231.38
deamidated (+1 Da)-0.26+0.98493%0.161.53

hydrolysed maleimide (+18 Da)

Retention offset from the main peak
-0.62 min on RG-2
Mass difference
+18.011 Da
Reported in
135 of 136 records (99 %)
Level when reported
median 0.35 %, max 2.10 %
Resolution requirement
Baseline-resolved on the reference method.

maleimide dimer

Retention offset from the main peak
+2.42 min on RG-2
Mass difference
+3644.975 Da
Reported in
130 of 136 records (96 %)
Level when reported
median 0.15 %, max 1.71 %
Resolution requirement
Baseline-resolved on the reference method.

mod-GRF (1-29) (−279 Da)

Retention offset from the main peak
-1.76 min on RG-2
Mass difference
-279.235 Da
Reported in
128 of 136 records (94 %)
Level when reported
median 0.23 %, max 1.38 %
Resolution requirement
Baseline-resolved on the reference method.

deamidated (+1 Da)

Retention offset from the main peak
-0.26 min on RG-2
Mass difference
+0.984 Da
Reported in
127 of 136 records (93 %)
Level when reported
median 0.16 %, max 1.53 %
Resolution requirement
Close-eluting — resolution below 1.2 is common and the peaks merge on short gradients.

Degradation pathways

Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.

  • maleimide ring hydrolysis to the unreactive maleamic acid — the defining stability problem
  • this hydrolysis is pH-driven and fast above pH 8