BPC-157 impurity profile
Every named species the index has reported alongside BPC-157, how often, at what level, and what its presence implies about the synthesis or the handling. Reporting threshold is 0.05 % area; anything below it is invisible to this page and is silently added to the main peak.
Median total impurities
0.85%
n=591
Median largest single
0.45%
n=591
Worst single observed
1.62%
n=591
Named species observed
6 / 6
n=6
| Named species | ΔRT (min) | Δ mass (Da) | Seen in | Median % area | Max % area |
|---|---|---|---|---|---|
| des-Gly truncate (−57 Da) | -0.34 | -57.021 | 84% | 0.13 | 1.32 |
| [D-Val]-diastereomer | -0.14 | mass-identical | 80% | 0.10 | 1.62 |
| pyroglutamate cyclisation (−17 Da) | +0.31 | -17.027 | 79% | 0.09 | 1.39 |
| acetylated N-terminus (+42 Da) | +0.48 | +42.011 | 78% | 0.09 | 1.56 |
| truncated (1-12) | -0.96 | -285.132 | 72% | 0.05 | 1.53 |
| BPC-157 arginine-salt artefact | -0.55 | mass-identical | 66% | 0.04 | 1.52 |
des-Gly truncate (−57 Da)
- Retention offset from the main peak
- -0.34 min on RG-2
- Mass difference
- -57.021 Da
- Reported in
- 495 of 591 records (84 %)
- Level when reported
- median 0.13 %, max 1.32 %
- Resolution requirement
- Baseline-resolved on the reference method.
[D-Val]-diastereomer
- Retention offset from the main peak
- -0.14 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 470 of 591 records (80 %)
- Level when reported
- median 0.10 %, max 1.62 %
- Resolution requirement
- A gradient long enough to separate it. No mass spectrometer will.
pyroglutamate cyclisation (−17 Da)
- Retention offset from the main peak
- +0.31 min on RG-2
- Mass difference
- -17.027 Da
- Reported in
- 465 of 591 records (79 %)
- Level when reported
- median 0.09 %, max 1.39 %
- Resolution requirement
- Baseline-resolved on the reference method.
acetylated N-terminus (+42 Da)
- Retention offset from the main peak
- +0.48 min on RG-2
- Mass difference
- +42.011 Da
- Reported in
- 459 of 591 records (78 %)
- Level when reported
- median 0.09 %, max 1.56 %
- Resolution requirement
- Baseline-resolved on the reference method.
truncated (1-12)
- Retention offset from the main peak
- -0.96 min on RG-2
- Mass difference
- -285.132 Da
- Reported in
- 428 of 591 records (72 %)
- Level when reported
- median 0.05 %, max 1.53 %
- Resolution requirement
- Baseline-resolved on the reference method.
BPC-157 arginine-salt artefact
- Retention offset from the main peak
- -0.55 min on RG-2
- Mass difference
- mass-identical — chromatography only
- Reported in
- 388 of 591 records (66 %)
- Level when reported
- median 0.04 %, max 1.52 %
- Resolution requirement
- A gradient long enough to separate it. No mass spectrometer will.
Degradation pathways
Degradants are a handling finding rather than a synthesis finding. A lot that shipped clean and arrived oxidised tells you about the courier, not the chemist.
- N-terminal pyroglutamate formation on storage of the free-base form
- Asp-Gly aspartimide during synthesis
- oxidative degradation is negligible — no Met, no Cys, no Trp